to set up the software for your use. This guide shows a workflow that uses application settings. BD FACSDiva Software Quick Reference Guide for the BD LSR II. Compensation Controls in the BD FACSDiva Software Reference Manual. • Default templates are now provided for certain instrument functions. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then Open the BD FACSDiva software and log on. Allow the.

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They can also include Sort Layouts, if you are running a sorter.

Getting Started with BD FACSDiva Software

See Using Experiment Layout on page See Uninstalling the Current Software on page You can force the gate to readjust by right-clicking on the gate boundary and choosing Recalculate from the shortcut menu. It will not run on Windows XP. The element manuap duplicated when the mouse button is released.

To add a blank global worksheet, leave the global worksheet template as Blank Analysis, and leave the Default global worksheet checkbox selected. Alternatively, double-click any worksheet element to locate the corresponding tube in the Browser.

This publication, or any part softwre, may not be reproduced or transmitted in any form or by any means, electronic, or Blackbaud, Inc. The pointer changes to green, and five green tabs appear in the Cytometer window. If any comments were saved with the template, they are shown in the box next to the list of templates. To move a window, drag the title bar to a new position on the screen.

If a label has been previously defined, choose it from the drop-down Label menu.



Getting Started Outlook vs. Page 4 Adjust the gates around the positive populations on the histogram for each stained control tube.

Software controls, consisting of up and down arrows and a slider bar, appear next to the value. It will remain selected until you select another button or press the Esc key.

Deleting Experiments To delete experiments, do the following: Getting Started The Excel Window u v w. Shortcut menus contain common commands that apply to the selected item. A new folder is created for each panel type. The icon turns referenec and the tube becomes the Active Tube in the Acquisition Dashboard. If additional assistance is required, contact your local BD Biosciences technical support representative or supplier.

Settings are then linked to the setup, so if you add a parameter that matches one in refrence setup, values for the matching parameter are updated facsiva. If there is a gated unstained population in the Unstained Control tube and a gated unstained population in the Stained Control tube, the population in the Stained Control tube will be used in the calculation.

To view worksheet options in the Inspector, click the background area of a worksheet. Threshold values can be adjusted from to approximatelyDensity Plot—graphical representation of two-parameter data where each axis displays the signal intensity of one parameter and colors indicate the number of events Density plots are similar to dot plots except colors are used to softwzre the accumulation of events density for events with the same signal intensity.

Label tubes in one of the following ways: See Publishing and Presenting Data on page 73 of this guide. Locked templates and any default templates provided with the software cannot be overwritten.

Adjusting the Size of Snap-To Gates When needed, you can adjust the size of a snap-to gate to capture a population s outlying events. Calculate compensation, and name the resulting setup. If you are not familiar with the Windows operating system, refer to the documentation provided with your computer.


Define selections by clicking Add Value, selecting the value in the Value field, and entering the required text. Data is listed by login name in a hierarchical view. Sharing Files and Whiteboards Your user role in a meeting determines your level of file sharing. For more information, see Importing Experiments on page Sage Software, Sage Software logos, and the Sage Software product and service names mentioned herein are registered More information.

The gate boundary is thicker to indicate that it is a snap-to gate. Tip If you forget to select the folder first, you can move an experiment using the Cut and Paste With Data commands.

FlowJo is a trademark of Tree Star, Inc. See the Troubleshooting section in the software or cytometer manuals.

See the next referencr. Stopping Time selection maintains the last entered value. Macintosh is a trademark of Rference Computer, Inc. Now, the button adds an experiment with a global worksheet, specimen, and tube. Before using this information and the product it supports, read the information in Notices More information.

The tabs are green to remind you that they show settings for the current tube. When you create a contour plot during acquisition, a density plot appears by default.

Polygon Gate button—allows a polygonal gate to be drawn around a population on a dot or contour plot.